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connie cepko  (Addgene inc)


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    Structured Review

    Addgene inc connie cepko
    Connie Cepko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+ert2creert2/pCAG-ERT2CreERT2+(Plasmid+%2313777)/pm41912583-157-12-14
    Average 93 stars, based on 90 article reviews
    connie cepko - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Construct:

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex.
    Article Snippet: .. To construct pNeuroD1-ERT2CreERT2 (#237402; Addgene), ERT2CreERT2 was digested with pCAG-ERT2CreERT2 (#13777; Addgene) and cloned into pNeuroD1-IRES-GFP, which D ev el o pm en t • A cc ep te d m an us cr ip t was digested with the same restriction enzymes. ..

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex
    Article Snippet: .. For the construction of pNeuroD1-ERT2CreERT2, ERT2CreERT2 was digested from pCAG-ERT2CreERT2 (Addgene, #13777) and cloned into pNeuroD1-IRES-GPF digested with the same restriction enzymes. pCAG-mCherry was constructed in the previous report ( ). ..

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex
    Article Snippet: .. To construct pNeuroD1-ERT2CreERT2 (Addgene plasmid #237402 ), ERT2CreERT2 was digested with pCAG-ERT2CreERT2 ( Addgene plasmid #13777 ) and cloned into pNeuroD1-IRES-GFP, which was digested with the same restriction enzymes. ..

    Clone Assay:

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex.
    Article Snippet: .. To construct pNeuroD1-ERT2CreERT2 (#237402; Addgene), ERT2CreERT2 was digested with pCAG-ERT2CreERT2 (#13777; Addgene) and cloned into pNeuroD1-IRES-GFP, which D ev el o pm en t • A cc ep te d m an us cr ip t was digested with the same restriction enzymes. ..

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex
    Article Snippet: .. For the construction of pNeuroD1-ERT2CreERT2, ERT2CreERT2 was digested from pCAG-ERT2CreERT2 (Addgene, #13777) and cloned into pNeuroD1-IRES-GPF digested with the same restriction enzymes. pCAG-mCherry was constructed in the previous report ( ). ..

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex
    Article Snippet: .. To construct pNeuroD1-ERT2CreERT2 (Addgene plasmid #237402 ), ERT2CreERT2 was digested with pCAG-ERT2CreERT2 ( Addgene plasmid #13777 ) and cloned into pNeuroD1-IRES-GFP, which was digested with the same restriction enzymes. ..

    Sequencing:

    Article Title: CellCousin2: an optimized system for partial ablation and tracing of regenerative lineages.
    Article Snippet: The fabp10a:ERT2CreERT2; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreERT2 in fabp10a:CreERT2; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) 23 with ERT2CreERT2 using EcoRI/NotI. .. The ERT2CreERT2 sequence was obtained fom pCAG-ERT2CreERT2, which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) 33. ..

    Article Title: Precise Progerin Targeting Using RfxCas13d: A Therapeutic Avenue for Hutchinson-Gilford Progeria Syndrome.
    Article Snippet: Unbin Chae, Hae-Jun Yang, Hanseop Kim, Seung Hwan Lee, Dong Gil Lee, Jeong Young Koo, Seung-min Ha, Seo-Jong Bak, Mina Joo, Hyun Hee Nam, Kyung-Seob Lim, Philyong Kang, Hee-Chang Son, You Jeong An, Young-Hyun Kim, In-Sung Song, Sang-Hee Lee, Hae Rim Kim, Sang-Mi Cho, Eun-Kyoung Kim, Ki-Hoan Nam, Kyung-Sook Chung, Jae-Yoon Kim, Seon-Yeop Kim, Seon-Kyu Kim, Seon-Young Kim, Dong-Seok Lee, Jin-Man Kim, Young-Ho Park, Sun-Uk Kim

    Plasmid Preparation:

    Article Title: CellCousin2: an optimized system for partial ablation and tracing of regenerative lineages.
    Article Snippet: The fabp10a:ERT2CreERT2; cryaa:mCerulean construct [abbreviated as fabp10a:ERCreER] was generated by replacing the CreERT2 in fabp10a:CreERT2; cryaa:mCerulean construct (Addgene plasmid # 230044 ; http://n2t.net/addgene:230044 ; RRID:Addgene_230044) 23 with ERT2CreERT2 using EcoRI/NotI. .. The ERT2CreERT2 sequence was obtained fom pCAG-ERT2CreERT2, which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) 33. ..

    Article Title: Pathophysiological significance of the p.E31G variant in RAC1 responsible for a neurodevelopmental disorder with microcephaly.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Article Title: Precise Progerin Targeting Using RfxCas13d: A Therapeutic Avenue for Hutchinson-Gilford Progeria Syndrome.
    Article Snippet: Unbin Chae, Hae-Jun Yang, Hanseop Kim, Seung Hwan Lee, Dong Gil Lee, Jeong Young Koo, Seung-min Ha, Seo-Jong Bak, Mina Joo, Hyun Hee Nam, Kyung-Seob Lim, Philyong Kang, Hee-Chang Son, You Jeong An, Young-Hyun Kim, In-Sung Song, Sang-Hee Lee, Hae Rim Kim, Sang-Mi Cho, Eun-Kyoung Kim, Ki-Hoan Nam, Kyung-Sook Chung, Jae-Yoon Kim, Seon-Yeop Kim, Seon-Kyu Kim, Seon-Young Kim, Dong-Seok Lee, Jin-Man Kim, Young-Ho Park, Sun-Uk Kim

    Knock-In:

    Article Title: Precise Progerin Targeting Using RfxCas13d: A Therapeutic Avenue for Hutchinson-Gilford Progeria Syndrome.
    Article Snippet: Unbin Chae, Hae-Jun Yang, Hanseop Kim, Seung Hwan Lee, Dong Gil Lee, Jeong Young Koo, Seung-min Ha, Seo-Jong Bak, Mina Joo, Hyun Hee Nam, Kyung-Seob Lim, Philyong Kang, Hee-Chang Son, You Jeong An, Young-Hyun Kim, In-Sung Song, Sang-Hee Lee, Hae Rim Kim, Sang-Mi Cho, Eun-Kyoung Kim, Ki-Hoan Nam, Kyung-Sook Chung, Jae-Yoon Kim, Seon-Yeop Kim, Seon-Kyu Kim, Seon-Young Kim, Dong-Seok Lee, Jin-Man Kim, Young-Ho Park, Sun-Uk Kim

    Expressing:

    Article Title: Multiplex generation and single cell analysis of structural variants in a mammalian genome
    Article Snippet: .. Recombinase expression plasmids used in this study are pCAG-iCre (Addgene #89573), pCAG-CreERT2 (Addgene #14797) and pCAG-ERT2CreERT2 (Addgene #13777) and pCAG-Bxb1 (pSP0722, modified from Addgene #51271). .. Between 200,000 and 350,000 cells were reverse transfected with the specific Cre plasmid using Lipofectamine 3000 (Thermo L3000001) in 6-well plates with the exception of the data shown in which was generated from a scaled-down version of this protocol performed in 12-well plates and 300 ng of Cre plasmid was transfected into ∼66000 cells.

    Modification:

    Article Title: Multiplex generation and single cell analysis of structural variants in a mammalian genome
    Article Snippet: .. Recombinase expression plasmids used in this study are pCAG-iCre (Addgene #89573), pCAG-CreERT2 (Addgene #14797) and pCAG-ERT2CreERT2 (Addgene #13777) and pCAG-Bxb1 (pSP0722, modified from Addgene #51271). .. Between 200,000 and 350,000 cells were reverse transfected with the specific Cre plasmid using Lipofectamine 3000 (Thermo L3000001) in 6-well plates with the exception of the data shown in which was generated from a scaled-down version of this protocol performed in 12-well plates and 300 ng of Cre plasmid was transfected into ∼66000 cells.



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    ert2creert2  (Addgene inc)
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    Genetic labeling of deep-layer neurons using in utero electroporation. (A) Experimental scheme used to label deep-layer neurons and trace their differentiation process. (B-D) <t>pNeuroD1-ERT2CreERT2,</t> pAAV-Ef1a-DIO-GFP and pCAG-mCherry plasmids (B,D) or pNeuroD1-IRES-GFP and pCAG-mCherry plasmids (C) were injected into the lateral ventricle of E12.0 embryos and electroporated. Pregnant mice were injected with tamoxifen at E13.0 (B,D). The brains were dissected out from the uterus at indicated stages and subjected to immunohistochemistry with anti-GFP, anti-RFP (mCherry) and anti-Ctip2 (B) antibodies. Nuclei were counterstained with Hoechst 33342. Images are representative of three samples. Scale bars: 400 µm (B); 200 µm (C,D). CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular/subventricular zone.
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    Image Search Results


    Genetic labeling of deep-layer neurons using in utero electroporation. (A) Experimental scheme used to label deep-layer neurons and trace their differentiation process. (B-D) pNeuroD1-ERT2CreERT2, pAAV-Ef1a-DIO-GFP and pCAG-mCherry plasmids (B,D) or pNeuroD1-IRES-GFP and pCAG-mCherry plasmids (C) were injected into the lateral ventricle of E12.0 embryos and electroporated. Pregnant mice were injected with tamoxifen at E13.0 (B,D). The brains were dissected out from the uterus at indicated stages and subjected to immunohistochemistry with anti-GFP, anti-RFP (mCherry) and anti-Ctip2 (B) antibodies. Nuclei were counterstained with Hoechst 33342. Images are representative of three samples. Scale bars: 400 µm (B); 200 µm (C,D). CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular/subventricular zone.

    Journal: Development (Cambridge, England)

    Article Title: In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex

    doi: 10.1242/dev.204564

    Figure Lengend Snippet: Genetic labeling of deep-layer neurons using in utero electroporation. (A) Experimental scheme used to label deep-layer neurons and trace their differentiation process. (B-D) pNeuroD1-ERT2CreERT2, pAAV-Ef1a-DIO-GFP and pCAG-mCherry plasmids (B,D) or pNeuroD1-IRES-GFP and pCAG-mCherry plasmids (C) were injected into the lateral ventricle of E12.0 embryos and electroporated. Pregnant mice were injected with tamoxifen at E13.0 (B,D). The brains were dissected out from the uterus at indicated stages and subjected to immunohistochemistry with anti-GFP, anti-RFP (mCherry) and anti-Ctip2 (B) antibodies. Nuclei were counterstained with Hoechst 33342. Images are representative of three samples. Scale bars: 400 µm (B); 200 µm (C,D). CP, cortical plate; IZ, intermediate zone; VZ/SVZ, ventricular/subventricular zone.

    Article Snippet: To construct pNeuroD1-ERT2CreERT2 (Addgene plasmid #237402 ), ERT2CreERT2 was digested with pCAG-ERT2CreERT2 ( Addgene plasmid #13777 ) and cloned into pNeuroD1-IRES-GFP, which was digested with the same restriction enzymes.

    Techniques: Labeling, In Utero, Electroporation, Injection, Immunohistochemistry